|
KMID : 1161420110140111456
|
|
Journal of Medicinal Food
2011 Volume.14 No. 11 p.1456 ~ p.1462
|
|
|
(?)-Epigallocatechin-3-O-Gallate Augments Pentobarbital-Induced Sleeping Behaviors Through Cl? Channel Activation
|
|
Park Kwang-Soon
Han Jin-Yi
Moon Dong-Cheul
Hong Jin-Tae
Oh Ki-Wan
|
|
|
Abstract
|
|
|
|
This experiment investigated whether (?)-epigallocatechin-3-O-gallate (EGCG) (5?20?mg/kg, p.o.) has hypnotic effects and/or enhances pentobarbital-induced sleeping behaviors and whether these effects are mediated by γ-aminobutyric acid (GABA) receptors. EGCG prolonged sleeping time induced by pentobarbital (42?mg/kg, i.p.) and reduced sleeping latency induced by pentobarbital similarly to muscimol (0.2?mg/kg, i.p.), a GABAA receptor agonist in mice. EGCG also increased sleeping rate and sleeping time when co-administered with pentobarbital (28?mg/kg, i.p.) at a subhypnotic dosage. In addition, EGCG and pentobarbital increased chloride (Cl?) influx in primary cultured cerebellar cells. EGCG and pentobarbital decreased GABAA receptors α-subunit expression and had no effect on the expression of β- and γ-subunits and of glutamic acid decarboxylase in the hippocampus of rats. In conclusion, the EGCG enhancement of Cl? influx may play an important role in pentobarbital-induced sleeping behaviors.
|
|
|
KEYWORD
|
|
|
γ-aminobutyric acidA receptors, chloride ion influx, (?)-epigallocatethin-3-O-gallate, glutamic acid decarboxylase, pentobarbital
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|